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Horiba Duetta Fluorescence and Absorbance Spectrometer Tutorial

Overview

UV-Vis

Duetta can be used as a fluorometer, as a UV-Vis-NIR spectrometer to measure absorbance, or as an instrument that measures true molecular fingerprints, which require the acquisition of fluorescence and absorbance, correcting for IFE in real time.

Index:

Standard Operating Protocol:

Start Up

Opening UV-Vis Lid

Operation

Select both
Click Acquire
Opening UV-Vis Lid Adding in cuvette

Data Analysis

Absorbance and Transmission

Shut down

Materials

Common failure modes

Manufacturer manuals

Exercises